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871645-20072

Annex 6 to the Grant Agreement

No ____of __,.____ 201

Environmental protection activities management plan

1. GENERAL INFORMATION ABOUT THE PROJECT AND PROJECT AREA

1.1. PROJECT DESCRIPTION

Project name: «Commercialization of bio-preparations for the protection of agroproducts at long-term storage»

Place of project realization: LLP “EcoSave” in rented production premises of LLP BIOTRON GROUP locating at: Republic of Kazakhstan, Akmola region, Stepnogorsk, Industrial zone 4, complex 7

The aim of the project: Introduction of integrated complex protection of agricultural products against microbial diseases and granary pests when stored in bio-preparation based vegetable stores.

Scope of the project and its activities sphere: Within the framework of the project, it is planned to produce environmentally friendly bio-preparations for the protection of agricultural products (potatoes, vegetables, fruits) during long-term storage:

- a preparation bacterial insecticidal Biokenbid-BMP;

- preparation bacterial fungicidal Biomikol-BMP.

The insecticidal preparation Biokenbide-BMP is obtained by deep cultivation of the culture of Bacillus thuringiensis variant thuringiensis pc. 620. The fungicidal preparation Biomikol-BMP is obtained by deep cultivation of the culture of Bacillus subtilis pc. IPM-215.

The final stage of production is the packing and labeling of the preparation and further storage and use. During the project implementation will be sold bio-preparations Biomikol-BMP and Biokenbid-BMP and services for scientific and technological support of the use of these preparations.

List of materials/ chemical substances which will be used during the project

If available, put CAS[1] number corresponding to a material / chemical substance[2]

Does it belong to hazardous materials according to the Chemical Substance Law?

Specify the material category according to the Chemical Substance Law (flammable, explosive, toxic, etc.)

Fodder yeast

8013-01-2

No

Does not belong to any category

Starch

9005-84-9

No

Does not belong to any category

Potassium phosphate monosubstituted

7778-77-0

No

Does not belong to any category

Potassium phosphate disubstituted

7778-77-0

No

Does not belong to any category

Magnesium sulphate hexahydrate

10034-99-8

No

Does not belong to any category

Manganese sulphate

10034-96-5

No

Does not belong to any category

Ammonium phosphate dibasic

7783-28-0

No

Does not belong to any category

Calcium chloride anhydrous

10043-52-4

No

Does not belong to any category

Sucrose

57-50-1

No

Does not belong to any category

Potassium chloride

7778-54-3

No

Does not belong to any category

Sodium chloride

7647-14-5

No

Does not belong to any category

Iron Sulfuric Oxide

7782-63-0

No

Does not belong to any category

Bacteriological peptone

73049-73-7

No

Does not belong to any category

Agar

9002-18-0

No

Does not belong to any category

1.2. LEGISLATION AND ADMINISTRATION

- National legislation: Environmental Code of the Republic of Kazakhstan (last amended as of November 16, 2015).

1.3. PROJECT DOCUMENTATION STATUS AND LICENSE AVAILABILITY

Certificate of land or property ownership: The sub-project is implemented on the production base of LLP "BIOTRON GROUP" by leasing the existing production sites.

Type of document or permit: the landlord is a manufacturer of bio-preparationcs and has a permit for emissions into the environment for objects of I, II and III categories until 2027.

- Permission for emissions into the environment of LLP BIOTRON GROUP (Annex 6.1)

- The contract for the lease of industrial premises between LLP BIOTRON GROUP and LLP "EcoSave " (Annex 6.2)

- Conclusion of the state ecological expertise on the draft standards for the emission of pollutants into the ambient air for LLP BIOTRON GROUP, Akmola region, Stepnogorsk (Annex 6.3)

- Conclusion on the assessment of the effects of the insecticide Biokenbid-BMP and fungicide Biomikol-BMP on the environment (Annex 6.4)

2. DESCRIPTION OF THE ENVIRONMENT (BASELINE STATE)

2.1 Placing a plot in relation to the surrounding area:

The enterprise LLP "BIOTRON GROUP" is located in the industrial zone of Stepnogorsk of Akmola region of the Republic of Kazakhstan, adjacent to the industrial site "Progress", and occupies an area of 1.67 hectares.

The nearest settlement, the village of Zavodskoy, is located at a distance of 1.5 km from the industrial site of the enterprise.

There are no posts for monitoring air pollution.

2.2 Brief description of the enterprise

The main activity of LLP "BIOTRON GROUP" is the production of preparations for vaccination of animals, preparations for biological treatment of oil contaminated soils, preparations for bioleaching of gold ore, disinfectant insecticide, and disinfectants for livestock.

On the leased territory of the considered site are located:

- Checkpoint;

- building for biopreparation production;

- mechanical workshop;

- block-modular boiler-house;

- Vivarium;

- garage.

There are standards of the organization for the production of bio-preparations Biokenbid-BMP and Biomikol-BMP:

- Bacterial insecticide preparation Biokenbid-BMP, (spore-crystalline complex of Bacillus thuringiensis culture, var. thuringiensis), ST 476-1902-46-LLP-02-2013.

- Bacterial fungicidal preparation Biomikol -BMP, (spore complex of culture Bacillus subtilis), ST 476-1902-46-TOO-03-2013.

In all rooms of the laboratory a set of technological clothes made of lint-free cloth is used to protect materials, semi-finished products and finished products from secondary contamination with microorganisms and mechanical particles emitted by personnel.

The complete set of technological clothes includes: underwear, overalls, cap, fully covering hair, calico bleached mask, leather-soled shoes, gloves. Contaminated clothing is collected in containers with a closing lid, installed in the sanitary gaps.

Personnel working in the laboratory undergo preliminary and periodic medical examinations and teach rules of conduct in clean rooms.

At least once every six months, the staff is instructed and at least once a year is checked on the requirements imposed on him when working in the premises of this class of cleanliness, knowledge of standard operational procedures and technological instructions.

In dressing rooms, the staff takes off their outer clothing, street shoes, removes personal belongings and puts on transitional clothes and slippers stored in another locker.

Laboratories have general exchange forced ventilation, which is integrated into one ventilation system and is carried out through fans of exhaust ventilation systems.

All used solutions are stored in a special laboratory vessel with tightly ground lids, which practically exclude evaporation.

Sanitary treatment of premises is carried out by modern disinfectants, which are delivered in plastic canisters, mixed with water, according to the instructions. There are no emissions of pollutants during sanitary treatment of premises.

The workplace and equipment are processed after the work is finished using a solution based on ethyl alcohol.

Quartz of laboratories is provided with bactericidal lamps.

Work schedule and staff of the enterprise. The operating time of the enterprise is 260 days a year. The staff of the enterprise is 7 people.

Electricity supply - the supply of electric energy is carried out from the existing electric grid in accordance with the lease agreement with LLP BIOTRON GROUP. Autonomous power supplies (diesel generator) are not available, and their installation is not planned.

Heat supply is provided from the boiler house.

Three similar solid fuel boilers are installed in the boiler house. Two boilers serve to heat the buildings of the enterprise in the autumn-winter period - 24 hours a day, 215 days a year. In summer, one boiler serves for the production of hot water for 8 hours a day, 150 days a year.

Boilers are equipped with smoke exhausters. On the pipe for dust and gas cleaning there is a cyclone BC 6 with a degree of purification of 85%.

The coal consumption for the needs of the boiler house is 380 tons / year.

2.3. Background pollution in the area of ​​the enterprise

Due to the fact that at present there are no regular observations of air pollution, the calculation of the dispersion of harmful substances, according to Kazhydromet, should be carried out taking into account the background concentrations presented in Table 1.2., established according to the conducted expeditionary surveys and analogical cities with a population of 10-50 thousand people. (RD 52.04, 186-89, M., 1991). In the area of ​​the enterprise location, the excess of background concentrations by controlled ingredients is not observed.

Table 2.3.1 Background concentrations of pollutants

Code of pollutants

Pollutant name

Concentration

mg / m3

2907

Dust (suspended matter)

0,2

0330

Sulphur dioxide

0,02

0337

Carbon monoxide

0,4

0301

Nitrogen dioxide

0,008

2.4 Water supply and sewerage

The withdrawal of water from surface sources for water supply of the enterprise and the discharge of sewage into open reservoirs are not carried out.

The water is used for household and drinking needs, washing the floors, feeding the heating system, production needs, the needs of the dining room and laboratories, watering the territory and green areas, firefighting needs.

For outdoor fire extinguishing in the territory is provided use of fire extinguishers. There is a fire station.

Sources of pollution of surface and groundwater are absent, the discharge of household wastewater is carried out in the existing settlement sewerage networks. Sewage treatment is not provided.

2.5 Hazard category of the enterprise

- The hazard category of the enterprise in accordance with the specific and qualitative composition of the emitted pollutants - 2.

- Class of sanitary hazard - in accordance with the "Sanitary and Epidemiological Requirements for the Establishment of the SPZ of Production Facilities" approved by the Government Decree of the Republic of Kazakhstan No. 237 of 20.03.2015 - the facility is classified as hazard class 3 with a SPZ of at least 300 m.

- The category of the facility for the significance and completeness of the environmental impact assessment in accordance with Article 40 of the Environmental Code of the Republic of Kazakhstan - 2.

Calculation of surface concentrations carried out on the program ECA v 1.7., showed that the existing situation on the border of the SPZ and the nearest residential zone, the concentration of harmful substances in the surface layer is not more than 1.0 MAC.

3. DETERMINATION OF POTENTIAL IMPACT

Biopreparations Biokenbid-BMP and Biomikol-BMP are are intended to be used in closed warehouses.

The effect of biopreparation Biokenbid-BMP, CS (spore-crystalline complex of Bacillus thuringiensis, variant thuringiensis) is the toxicity of the species-produced species-specific toxin to barn pests.

According to the base of the International Register of the UN PTC of the American base "iris", the basis of the insecticidal biopreparation Baillus variant thuringiensis is safe for vertebrates; practically insoluble in water, dissolves in the intestines of sensitive insect species.

The effect of biopreparation Biomikol-BMP, CS (spore complex of Bacillus subtilis culture) against microbial diseases of agricultural products is manifested due to antagonistic and antibiotic properties of metabolites of Bacillus subtilis culture.

According to the base of the International Register of the UN PTC of the US base "iris", the basis of the insecticidal biological product Bacillus subtilis is nontoxic for warm-blooded animals; quickly decomposes in sunlight, it is poorly soluble in water.

The main potential impact on the environment is the emission of carbon dioxide due to the use of coal for heating production facilities based on LLP BIOTRON GROUP. The Lessor takes all necessary mandatory payments for carbon dioxide emissions into the atmosphere.

The project does not imply an increase in production capacity and additional heating of premises, also an increase in the amount of used fuel.

3.1 Characteristics of the enterprise as a source of atmospheric pollution

The considered object is a source of pollution of the atmosphere by emissions of pollutants formed as a result of technological processes associated with the operation of the boiler house.

The sources of emissions of harmful substances into the atmosphere are:

• Boiler house (a source number 0001). Three similar boilers with natural gas are installed in the boiler house. Two boilers serve to heat the buildings of the enterprise in the autumn-winter period - 24 hours a day, 168 days a year. One boiler serves for the production of hot water supply all year round for 8 hours a day. Each boiler has its own separate pipe. The pipes are routed to the roof. There are no installations for dust and gas purification. Only natural gas is used as fuel. The main hazards are: inorganic dust (2908), carbon monoxide, nitrogen dioxide, nitrogen oxide, sulfurous anhydride;

Fuel for the boiler house is stored in an open area of ​​25 m2 (source 6001). In the winter, dust from the coal store does not occur due to the snow cover of the warehouse (high humidity of the ash). When coal is received, inorganic dust is released (2909);

Ash and slag removal is carried out manually. The ash is stored in an open area (source 6002), the area of ​​which is 15 m2 and, as accumulation, is exported to a landfill or sold to the public. In the winter, dust from the ash warehouse does not occur due to the snow cover of the warehouse (high moisture of the ash). With the reception of ash, inorganic dust is released (2908).

List of pollutants emitted into the atmosphere to the existing situation.

Table 3.1.1 List of pollutants emitted into the atmosphere to the existing situation:

3.2 Calculation of hazard category

To determine the hazards of enterprises are used data on emissions of pollutants into the atmosphere.

The enterprise's hazard category (EHC) is calculated by the formula:

n i ai

EHC = ∑ (--------) , where

i =1 MPCi

i - mass emission i – of that substance, ton/year

MPC i - average daily maximum permissible concentration i of that substance mg/ m 3

n amount of pollutants emitted by the enterprise

ai - a dimensionless constant that allows to correlate the degree of harmfulness i- of the substance with the harmfulness of sulfur dioxide.

The values of ai for substances of different hazard classes

Constant

Hazard Class

1

2

3

4

ai

1,7

1,3

1,0

0,9

The value of the EHC is calculated under the condition when Mi / MPCi> 1. When Mi / PDKi <1, the EHC values are not calculated and equated to zero. To calculate the EHC in the absence of daily average values of maximum permissible concentrations are used the values of the maximum one-time MPCs, ASLE or the values of the maximum permissible concentration of the working area, reduced by a factor of 10.

For substances for which there is no information on MPC or ASLE, the values of EHC are equated to the mass of emissions of these substances. By the size the enterprise's hazard category is divided into four categories of danger. The boundary conditions for dividing the enterprise into hazard categories are given in the table:

Hazard category of the enterprise

The value of EHC

I

EHC > 106

II

106 > EHC > 104

III

104 > EHC > 103

IV

EHC < 103

The calculation of the EHC is given in the List of pollutants emitted into the atmosphere to the existing situation.

3.3 Calculation and analysis of values of surface concentrations of pollutants

Calculation of surface concentrations on the existing position was performed on the software package ECA v 1.7.

The initial data accepted for calculation:

1. The calculated rectangle is accepted 500x500m and allows to determine the zone of influence of the enterprise on the environment;

grid pitch 50x50m;

scale 1: 3800;

The calculation is made in the factory coordinate system, the center of the calculated rectangle is the geometric center of the site with the coordinates X = 0m, Y = 0m;

the angle between the axis OX and the direction to the north is 90 degrees;

for the control zone, a sanitary protection zone is established, which is at least 300 m and the nearest settlement, Zavodskoye village is located at a distance of 1.5 km from the industrial site of the enterprise;

* Terrain coefficient is adopted according to RND 211.2.01.01-87 and is equal to 1;

The calculation is based on the maximum estimated emissions from all emission sources, taking into account the simultaneous operation of the equipment in the winter, taking into account the boiler house operation;

Calculations are based on the maximum estimated emissions from all sources, taking into account the simultaneity of work on all substances.

Conclusions:

Analysis of the results of calculations on the existing situation shows that at the border of the SPZ and the nearest residential house the maximum concentration for all substances does not exceed 1 MPC.

Consequently, the work of the enterprise does not have a significant impact on air pollution and additional measures to reduce emissions of pollutants are not required. Based on the results of the impact assessment, a Permit for emissions into the environment was issued. (Annex 6.1)

3.4 Ecological characteristics of pesticides

3.4.1 The active substance of biopreparation Biokenbid-BMP Bacillus thuringiensis variant thuringiensis and the products of its vital activity (δ-endotoxin)

3.4.1.1 Environmental behavior

Distribution, persistence, mobility and reproduction

The soil

Conditions and methods

Indicators

Data sources

Distribution

Bacteria are actively sorbed in the upper horizon of the soil.

1. http://sitem.herts.ac.uk/aeru/footprint/en/Reports/57.htm

2. . Wondimagegnehu, S. Megh Uptake, translocation, and metabolism of [14C] thuringiensin (beta-exotoxin) in com //Journal of Agricultural and Food Chemistry. 1989. V. 37. P. 481-483.

3. M. Ghassemi, et al., Environmental fates and impact of major forest use pesticides// Environmental Protection Agency. 1981. Washington, D.C.

4. Reregistration eligibility decision Bacillus thuringiensisHEPA. Washington, D.C.

5. Kozhevin P.A. Microbial populations in nature, - M.: P.

Persistence

δ-endotoxin:

DT50 = 2.7 days

Mobility and reproduction

δ-endotoxin:

Kos = 5000 ml / g

The reproduction of microorganisms on plants and in the soil does not occur. Solar activity is the main reason for the inactivation of B. thuringiensis spores. The humid climate, like solar radiation, intensifies the inactivation of the spores.

Bacteria are inactive in the soil, unstable, do not multiply actively. Endotoxin is rapidly degraded by soil microorganisms, immobile in the soil. It is expected that the maximum abundance of B. thuringiensis in the upper 10 cm layer of soil does not exceed 1.25 x 105 CFU/g soil. This value is 4 orders lower than the average total bacterial content in 1 g of soil, and the number is comparable to the native content of the native strain.

The risk of soil contamination with B. thuringiensis and products of vital activity when applying the preparation of Biokenbid-BMP is considered to be low.

Conditions and methods

Indicators

Data sources

Distribution

. thuringiensis is not an inhabitant of water bodies, development of a spore into water is unlikely. δ-endotoxin can be active for 48 hours, the main toxin inactivation occurs due to the formation of complexes with suspended organic matter.

1. http://extoxnet.orst.edu/pips/bacillus.htm

2. J. Jenkins History of . thuringiensis, ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY MEMO Department of Agricultural Chemistry, Oregon State University, 1992

Persistence

Mobility and reproduction

Culture can live in wetland, however, water is not its place, since V. thuringiensis is a strict aerobic. Reproduction in water is excluded. It is expected that the maximum possible amount of B. thuringiensis in the surface water body (standard 2% demolition, reservoir 300000 l, model Step 1) will not exceed 106 CFU/l, which is below the established fishery MPC for B. thuringiensis in 107 CFU/l (recalculation of the drug Bitoksibatsillin, P).

The risk of contamination of water bodies, land and groundwaters by the culture of B. thuringiensis and its products of vital activity when using the preparation Biokenbid-BMP is assessed as low.

Air

Conditions and methods, indicators

Data sources

1. Indication of Bacillus thuringiensis in the air for 17 days after intensive use

2. Solar activity is the main cause of inactivation of Bacillus thuringiensis spores

1.. Wondimagegnehu, S. Megh Uptake, translocation, and metabolism of [14C] thuringiensin (beta-exotoxin) in corn //Journal of Agricultural and Food Chemistry.1989. V. 37. P. 481-483.

2.Reregistration eligibility decision Bacillus thuringiensis H. Washington, D.C.

High molecular toxins are non-volatile compounds, when sunlight acts B. thuringiensis and spores are inactivated.

The risk of air pollution by the culture of B. thuringiensis and toxins when using Biokenbid-BMP is estimated as low.

Studies of a possible fate in food chains are not required, since the titer of spores of microorganism cells introduced into the soil is comparable to the natural content.

3.4.2 The active substance of biopreparation Biomikol-BMP Bacillus subtilis and products of its life

3.4.2.1 Environmental behavior

Distribution, persistence, mobility and reproduction

The soil

Conditions and methods

Indicators

Data sources

Distribution

Bacteria are actively sorbed in the upper horizon of the soil

Biopesticide registration action document Bacillus subtilis Strain QST 713 / U.S. Environmental Protection Agency

.. ,- .:-.

http://rupest.ru/ppdb/bacillus-subtilis.html#5

Persistence

DT50=150 days

Mobility and reproduction

Kos = 5000 ml / g

Solar activity is the main reason for the inactivation of B. subtilis spores. The humid climate, like solar radiation, intensifies the inactivation of the spores.

Bacillus subtilis is a ubiquitous microorganism, but primarily a soil inhabitant. As vegetative growth slows down as nutrient sources decline, this species competes poorly for limited resources, and the B. subtilis population will compete in the natural microflora.

The risk of soil contamination with . subtilis and products of vital activity when using the preparation Biomikol-BMP is estimated as low.

Water

Conditions and methods

Indicators

Data sources

Distribution

Bacillus subtilis is a ubiquitous bacteria commonly found in various ecological niches including water that do not have pathogenicity in the environment. In addition, there are other strains of B. subtilis that are registered as microbial pesticides.

https://www3.epa.gov/pesticides/chem_search/reg_actions/registration/decision_PC-006479_9-Aug-06.pdf

Persistence

Mobility and reproduction

The risk of contamination of water bodies, land and groundwaters by the culture of . subtilis and its products of vital activity when using the preparation Biomikol-BMP is assessed as low.

Air

Conditions and methods, indicators

Data sources

1. Bacillus subtilis is a ubiquitous bacteria commonly found in various ecological niches including water that do not have pathogenicity in the environment. In addition, there are other strains of B. subtilis that are registered as microbial pesticides.

2. Solar activity is the main cause of inactivation of Bacillus subtilis

https://www3.epa.gov/pesticides/chem_search/reg_actions/registration/decision_PC-006479_9-Aug-06.pdf

High molecular toxins are non-volatile compounds, when sunlight acts on . Subtilis, it is inactivated.

The risk of air pollution by the culture of . subtilis and toxins when using Biokenbid-BMP is estimated as low.

Studies of a possible fate in food chains are not required, since the titer of spores of microorganism cells introduced into the soil is comparable to the natural content.

3.4.3 Risk assessment of the preparation use

3.4.3.1 Risk assessment of use of preparation Biokenbid-BMP

Terrestrial vertebrates

Mammals

Type of toxicity, conditions and methods

Indicators

Data sources

Acute oral toxicity

Rats

SS 32644-2014 "Method for determining the class of acute toxicity"

. thuringiensis and δ-endotoxin:

LD50> 0.06x1011-5.5x1011 CFU/ kg (equivalent to LD50 medium> 5600 mg / kg of preparation)

Reports on the expert evaluation of EFSA for preparations based on B. thuringiensis strains

Pathogenicity

There are no visual signs of infectivity and pathogenicity during animal necropsies

Infectivity

Bioinsecticide Biokenbid-BMP is practically non-toxic preparation for mammals (not classified by danger). . thuringiensis is not a pathogenic microorganism and can not infect mammals.

Birds

Type of toxicity, conditions and methods

Indicators

Data sources

Acute oral toxicity, mallard

Guideline No. 223 analogue of SS 33059-2014 "Birds: Acute Oral Toxicity Test"

. thuringiensis and δ-endotoxin: LD50 > 1,8 1010 - 6x1010 CFU/ kg

(equivalent LD50 medium> 6200 mg / kg of preparation)

Reports on the expert evaluation of EFSA for preparations based on B. thuringiensis strains

Pathogenicity

There are no visual signs of infectivity and pathogenicity during animal necropsies

Infectivity

Bioinsecticide Biokenbid-BMP is practically non-toxic preparation for birds (not classified by danger). . thuringiensis is not a pathogenic microorganism and can not infect birds.

The use of the preparation Biokenbid-BMP (spore-crystalline complex of the Baillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ ml) is associated with a low risk for mammals and birds.

Aquatic organisms

Type of toxicity, conditions and methods

Indicators

Data sources

Oncorhynchus mykiss, 96hours

Fish

. thuringiensis and δ-endotoxin:

LC50 >8,7109 - 4,61010 CFU/l (equivalent EC50med> 547 mg / L of preparation)

Zooplankton

. thuringiensis and δ-endotoxin:

50>2,4107 - 4,2109 CFU/l (equivalent EC50 med. >42 mg/l of preparation) NOEC=3,8x105-1,7x108 CFU/l (equivalent tNOEC= 1,7 mg/l of preparation)

Seaweed

. thuringiensis and δ-endotoxin EC50>2,3x109-5,2x109 CFU/l

(equivalent 50 med.> 75 mg/l of preparation)

1. Reregistration eligibility decision Bacillus thuringiensis HEPA.

Washington, D.C.

Daphnia magna, 48 hours

Reports on the expert evaluation of EFSA for preparations based on B. thuringiensis strains

Daphnia magna, 21 days

Selenastrum capricornotum, 72hours

Guideline No. 203 Analog of SS 32473-2013 "Determination of Acute Toxicity for Fish"

Guideline No. 452 Analog of SS 32536-2013 "Determination of Acute Toxicity for Daphnia

giensis

3 .http://extoxnet.orst. edu/pips/bacillus.htm

Bioinsecticide Biokenbid-BMP is practically non-toxic preparation for fish (not classified by danger). . thuringiensis is not a pathogenic microorganism and can not infect aquatic organisms.

The use of the preparation Biokenbid-BMP (spore-crystalline complex of the Baillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ml) is associated with a low risk for aquatic life and fauna.

Honey bees

Type of toxicity, conditions and methods

Indicators

Data sources

Acute oral and contact toxicity

Guideline No. 214 analog of SS 33039-2014 "Honeybees: test for acute toxicity"

. thuringiensis and δ-endotoxin:

LD50> 2105 - 3106 CFU/bee (equivalent LD50 =4-60 mcg / preparation of the bee)

1. http://sitem.herts.ac.uk /aeru/bp db/Rep orts/5 7. htm

2.

Reports on the expert evaluation of EFSA for preparations based on B. thuringiensis strains

Bioinsepticide Biokenbid-BMP is mildly toxic to bees (hazard class 2).

The use of the drug Biokenbid-BMP (spore-crystalline complex of Baillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ml) is associated with a risk for bees.

Earthworms

Type of toxicity, conditions and methods

Indicators

Data sources

Acute oral toxicity

Eisenia fetida, 14 days

Guideline No. 207 Analog of SS 33036-2014 "Determination of Acute Toxicity for Earthworms"

. thuringiensis and δ-endotoxin:

LC5o>3x1010 CFU/kg (equivalent LC5o>625 mg/kg of preparation)

Reports on the expert evaluation of EFSA for preparations based on B. thuringiensis strains

Bioinsepticide Biokenbid-BMP is a weakly toxic preparation for earthworms (3rd class of danger).

The use of the preparation Biokenbid-BMP (spore-crystalline complex of Baillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ml) is associated with a low risk for earthworms.

Soil microorganisms

The strain of . thuringiensis is isolated from natural soil, is a typical herbivore, is also widely found in plant rhizosphere. Risks of negative influence of . thuringiensis on native microflora are absent.

Ecological hazard of the drug Biokenbid-BMP (spore-crystalline complex of Baillus thuringiensis variant thuringiensis spore titer is not less than 109 CFU/ml).

The ecological hazard of the pesticide is manifested in its ability to pollute the environment (soil, water and air) and adversely affect non-target (useful) types of organisms. Below are the classes of properties and ecological hazard of the pesticide, established on the basis of the above data in the table.

Classes of properties and environmental hazards of . thuringiensis and the preparation Biokenbid-BMP

Object/property

Hazard statement

Hazard class

Soil

Resistance

Unstable

-

Soil/water

Mobility

Fixed

-

Air

Volatility

Non-volatile

-

Mammals

Practically non-toxic

Not classified2

Aquatic

Fish

Practically non-toxic

Not classified3

Zooplankton

Harmful

33

organisms

Seaweed

Harmful

33

Earthworms

Medium Toxic

33

Birds

Acute toxicity

Practically non-toxic

Not classified 1

Bees

Medium Toxic

21

Risk of use

1 - Guidance on classifications of environmental hazards of pesticides. B. Vyazemy, ARSRIP, 2010,17p.

2 - SS 32419-2013. Classification of the hazard of chemical products. General requirements.

3 - SS 32424-2013. Classification of the hazard of chemical products on the impact on the environment. Basic features.

3.4.3.2 Risk assessment of the preparation Biomikol-BMP

Terrestrial vertebrates

Mammals

Type of toxicity, conditions and methods

Indicators

Data sources

Acute oral toxicity

Rats

SS 32644-2014 "Method for determining the class of acute toxicity"

Acute oral LD50> 5000 mg / kg, L3 Rat.

1. http://rupest.ru/ppdb/bacillus-subtilis.html#5

2. https://www3.epa.gov/pesticides/chem_

search/reg_actions/registration/decision_PC-006479_9-Aug-06.pdf

Pathogenicity

There are no visual signs of infectivity and pathogenicity during animal necropsies

Infectivity

Bioinsecticide Biomikol-BMP is practically non-toxic preparation for mammals (not classified by danger). . subtilis is not a pathogenic microorganism and can not infect mammals.

Birds

Type of toxicity, conditions and methods

Indicators

Data sources

Acute oral toxicity

Guideline No. 223 Analog of SS 33059-2014 "Birds: Acute Oral Toxicity Test"

B. subtilis

Bird oral LD50 exceeds 5000 mg /kg of body weight.

Foster, J.; Grimes, J.; Beavers, J. (1998) An Avian Oral

Pathogenicity and Toxicity Study in the Northern Bobwhite

Pathogenicity

There are no visual signs of infectivity and pathogenicity during animal necropsies

Infectivity

Bioinsecticide Biomikol-BMP is practically non-toxic preparation for birds (not classified by danger). . subtilis is not a pathogenic microorganism and can not infect birds.

The use of the preparation Biomikol-BMP (spore-crystalline complex of the Baillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ml) is associated with a low risk for mammals and birds.

Aquatic organisms

Type of toxicity, conditions and methods

Indicators

Data sources

Guideline No. 203 Analog of SS 32473-2013 "Determination of Acute Toxicity for Fish"

Guideline No. 452 Analog of SS 32536-2013 "Determination of Acute Toxicity for Daphnia"

Fish

. subtilis:

The 30-day LC50 in rainbow trout was calculated as 1.4 × 107 cfu ml. in water, Mortality and the absence of an observed level of side effects were not observed 1.7 × 106 cfu / ml.

Zooplankton

. subtilis:

50>2,1107 - 4,7109 CFU/l (equivalent to 50 med. >42 mg/l of preparation) CFU/l)

Seaweed

. subtilis:

EC50>2,5x109-5,5x109 CFU/l

(equivalent to 50 med.> 75 mg/l of preparation)

Drottar, K.; Flaggs, R.; Krueger, H. (2001) QST 713 Technical: A

Five-Concentration Toxicity and Pathogenecity Test with the Rainbow Trout

Bioinsecticide Biomikol-BMP is practically non-toxic preparation for zooplankton and seaweed (not classified by danger). . subtilis is not a pathogenic microorganism and can not infect aquatic organisms.

The use of the preparation Biomikol-BMP (spore-crystalline complex of the Baillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ml) is associated with a low risk for aquatic life and fauna.

Honey bees

Type of toxicity, conditions and methods

Indicators

Data sources

Acute oral and contact toxicity

Guideline No. 214 analog of SS 33039-2014 "Honeybees: test for acute contact toxicity"

. subtilis:

LD50> 100,000, is the application rate of 400X and> 800X EEC, from ground application of B. subtilis from the current usage rate, no danger is expected for honey bees

Jimenez, D.; Richards, K. (2004) Evaluation of the Dietary

Effect(s) of QST 713 Technical Powder on Larval Honeybee Development (Apis

mellifera L.)

Bioinsepticide Biomikol-BMP is mildly toxic to bees (hazard class 4).

The use of the preparation Biomikol-BMP (spore-crystalline complex of Baillus subtilis spore titer of not less than 109 CFU/ml) is associated with a low risk for bees.

Earthworms

Type of toxicity, conditions and methods

Indicators

Data sources

Acute oral toxicity

Eisenia fetida, 14 days

Guideline No. 207 Analog of SS 33036-2014 "Determination of Acute Toxicity for Earthworms"

. subtilis:

LC50> 1000

PREPARATION / KG DRY SOIL

EQUIVALENT NOEC 316 Mg / KG DRY MASS

Review report for the active substance Bacillus subtilis

Bioinsepticide Biomikol-BMP is a weakly toxic preparation for earthworms (3rd class of danger).

The use of the preparation Biomikol-BMP (spore-crystalline complex of Baillus subtilis spore titer of not less than 109 CFU/ml) is associated with a low risk for earthworms.

Soil microorganisms

The strain of . subtilis is isolated from natural soil, is a typical herbivore, is also widely found in plant rhizosphere. Risks of negative influence of . subtilis on native microflora are absent.

Ecological hazard of the drug Biomikol-BMP (spore-crystalline complex of Baillus subtilis spore titer is not less than 109 CFU/ml).

The ecological hazard of the pesticide is manifested in its ability to pollute the environment (soil, water and air) and adversely affect non-target (useful) types of organisms. Below are the classes of properties and ecological hazard of the pesticide, established on the basis of the above data in the table.

Classes of properties and environmental hazards of Baillus subtilis and the preparation Biomikol-BMP

Object/property

Hazard statement

Hazard class

Soil

Resistance

Unstable

-

Soil/water

Mobility

Fixed

-

Air

Volatility

Non-volatile

-

Mammals

Practically non-toxic

Not classified2

Aquatic

Fish

Practically non-toxic

Not classified3

Zooplankton

33

organisms

Seaweed

33

Earthworms

Medium Toxic

33

Birds

Acute toxicity

Practically non-toxic

Not classified 1

Bees

Practically non-toxic

Not classified 1

4 - Guidance on classifications of environmental hazards of pesticides. B. Vyazemy, ARSRIP, 2010,17p.

5 - SS 32419-2013. Classification of the hazard of chemical products. General requirements.

6 - SS 32424-2013. Classification of the hazard of chemical products on the impact on the environment. Basic features.

3.5 Environmental risk of preparation use

Environmental risk is "the probability of an event that has adverse consequences for the natural environment ...". For a pesticide, this concept can be interpreted as the likelihood of its ecological danger (pollution of natural environments and toxicity) under real environmental conditions and application regulations.

3.5.1 The environmental risk of using the preparation Biokenbid-BMP (spore-crystalline complex Baillus thuringiensis variant thuringiensis spore titer of at least 109 cfu/ml) and its management (limitations of use)

According to the data on the standard assessment of the deterministic environmental risk of a pesticide, the use of the preparation Biokenbid-BMP (spore-crystalline complex Baillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ml) is associated with low levels of risks of pollution of natural media and negative impact on non-target (useful) species of organisms other than bees.

Conclusions:

The list and volume of documentation of the pesticide Biokenbid-BMP (spore-crystalline complex of the bacillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ml) satisfy the registration requirements in force in the Republic of Kazakhstan. Methods and conditions for conducting experiments, tools for assessing environmental hazards and pesticide risk meet national and internationally accepted standards. It has been established that the use of the pesticide Biokenbid-BMP (spore-crystalline complex of Bacillus thuringiensis variant thuringiensis spore titer of not less than 109 CFU/ml) in accordance with the regulations is associated with low environmental risk. Based on the foregoing, the biopreparation was registered for use in the Republic of Kazakhstan.

3.5.2 The environmental risk of using the preparation Biomikol-BMP (spore-crystalline complex Baillus subtilis spore titer of at least 109 CFU/ml) and its management (limitations of use)

According to the data on the standard assessment of the deterministic environmental risk of a pesticide, the use of the preparation Biomikol-BMP (spore-crystalline complex Baillus subtilis spore titer of not less than 109 CFU/ml) is associated with low levels of risks of pollution of natural media and negative impact on non-target (useful) species of organisms other than bees.

Conclusions:

The list and volume of documentation of the pesticide Biomikol-BMP (spore-crystalline complex of the Baillus subtilis spore titer of not less than 109 CFU/ml) satisfy the registration requirements in force in the Republic of Kazakhstan. Methods and conditions for conducting experiments, tools for assessing environmental hazards and pesticide risk meet national and internationally accepted standards. It has been established that the use of the pesticide Biomikol-BMP (spore-crystalline complex of Baillus subtilis spore titer of not less than 109 CFU/ml) in accordance with the regulations is associated with low environmental risk. Based on the foregoing, the biopreparation was registered for use in the Republic of Kazakhstan.

4. MITIGATION AND MONITORING PLAN

4.1 Mitigation plan

According to the enterprise's reconstruction, a change in technology, the release of new products for the next 5 years is not planned.

According to the results of calculations of surface concentrations from sources of emission of harmful substances, exceeding of the limit norms is not observed, measures to reduce emissions are not required and have not been developed.

In order to comply with the regulatory values of air pollutant emissions, it is proposed to implement general measures.

Annual instrumental control over boiler house emissions;

Observe the technological regulations of the laboratories;

Timely repair of the asphalt covering of the territory, regular watering in the summertime;

Separate collection and utilization of industrial waste;

The collection and storage (before removal) of solid domestic waste is carried out in containers located on a site with a hard surface.

4.1 Measures to reduce pollutant emissions for the period of unfavourable meteorogical conditions

The nearest settlement, the village of Zavodskoy, is located at a distance of 1.5 km from the industrial site of the enterprise. Since the height of the pipes of gas boilers in the boiler room exceeds the height of the nearest residential buildings, there is no impact.

The considered object is located outside the boundaries of water protection zones and strips of surface water bodies.

1. Calculation of the dispersion of pollutants showed that the existing position maximum surface concentrations for all ingredients on the boundary of the SPZ and the nearest housing do not exceed 1 MAC.

The hazard category of the enterprise in accordance with the specific and qualitative composition of the emitted pollutants - 2;

Class of sanitary hazard - in accordance with the "Sanitary and Epidemiological Requirements for the Establishment of the SPZ of Production Facilities" approved by the Government Decree of the Republic of Kazakhstan No. 93 of 17.01.2012 - the facility is classified as hazard class 3 with a SPZ of at least 300 m. of the production of finished dosage forms (without manufacturing components);

The category of the facility for the significance and completeness of the environmental impact assessment in accordance with Article 40 of the Environmental Code of the Republic of Kazakhstan - 2.

2. In connection with the fact that currently there are no regular observations of air pollution, the calculation of the dispersion of harmful substances, according to Kazhydromet, should be carried out taking into account the background concentrations established by the data of conducted expeditionary surveys and analogical cities with a population of 10-50 thousand . (Manual for air pollution control 52.04, 1.86-89, Moscow,1991). The background concentrations are as follows: for dust - 0.2 mg / m3, for sulfur dioxide - 0.02 mg / m3, for nitrogen dioxide - 0.008 mg / m3 and for carbon oxide - 0 , 4 mg / m3. In the area of ​​enterprise sites location, the excess of background concentration on controlled ingredients isn't observed.

3. Provision of water needs is carried out from own well. The site of the water intake well has a fenced zone of sanitary protection. The well is equipped with a water meter. As a result of activities, household and industrial wastewaters are formed, sewage treatment is not provided.

Discharge of sewage is carried out in the existing settlement sewage networks. Sewage treatment is not provided.

4. Technological installations having electric motors are installed on vibration-proof stands.

Based on the foregoing, it can be concluded that the enterprise does not have a significant impact on the ecological situation in the district.

4.1.2 Strategy to reduce the risk of using preparations Biokenbid-BMP and Biomikol-BMP.

The use of preparations "Biokenbid-BMP" and "Biomikol-BMP" is practically harmless for bees, but can have an adverse effect on bees. The grant applicant is a producer, but not a user of a biopesticide.

The following measures will be undertaken to mitigate possible impacts:

a) the regulations on the use of Biokenbid-BMP and Biomikol-BMP will be developed, where beekeeping activities will be indicated

- to work with "Biokenbid-BMP" and "Biomikol-BMP" in closed premises, as these preparations are designed for processing storage space. However, the following should be considered:

- work at a wind speed of less than 5-6 m / s (in order to avoid the transfer of the pesticide by wind);

- conduct a survey of the area and make sure that there are no wild bees within a radius of 1-2 km from the processing site;

- When finding nesting grounds, contact specialists to limit the flying of bees to 3-5 days after treatment;

- to warn honey producers about the forthcoming processing and restrictions of bees flying for 3-5 days;

b) when marking a pesticide, will be used a special sign "dangerous for bees"

c) the label will contain the inscription "For the use of the pesticide outside the regulations of use and the resultant death of bees is the responsibility of the user"

Note: when selling preparations, we can not control their use. We are required to notify the user of the risk, and to offer information to minimize the impact by limiting the extent or scale of the activity and its implementation.

The emergence of risk and subsequent compensation for environmental damage are the responsibility of the consumer.

Mitigation plan

The stage of the project is expected to implement activities of a general nature.

Activities

Expected impact on the environment

The proposed mitigation measure

Responsibility for the implementation of mitigation measures

The period of implementation of mitigation measures

1. Compliance with the regulatory values of air pollutant emissions (According to the Appendix - Permission for emissions into the environment)

According to the results of calculations of surface concentrations from sources of emission of harmful substances, exceeding the limit values are not observed, no reduction measures are required

Observe the technological regulations of the boiler house and production sites

Administration of LLP "BIOTRON GROUP"

Heating period

Always

Timely repair of the asphalt covering of the territory, regular watering in the summertime

Summer period

Separate collection and utilization of industrial waste

Always

The collection and storage (before removal) of solid domestic waste is carried out in containers located on a site with a hard surface

Always

Development of a memo on the hazard of toxicity risk for bees with mandatory insertion into any unit of sold product (package)

- processing in closed rooms

- at a wind speed of <5-6 m / s

- boundary protection zone for bees> 1-2 km

- Limiting the flight of bees> 6-12 hours

Administration of LLP EcoSave

Explanations throughout the work of the project and beyond.

2. Limited preparation use

Practically not dangerous for bees.

Development of the sign "Caution! Harmful for bees" on the label.

To bring to the users of the preparation the responsibility for the safety of bees

Employees of LLP "EcoSave":

Zhappar N.,

Shaykhutdinov V.

Throughout the work of the project and beyond.

Work stage

3. The process of obtaining biopreparation

Emissions to the atmosphere

For the personnel of the laboratories will be conducted trainings and briefings on methods to minimize emissions to the atmosphere.

Will be prepared a list of actual and potential emission sources in the laboratory (smoke and vent pipes, etc.)

Zhappar N.,

Shaykhutdinov V.

Explanations throughout the work of the project and beyond.

Handling of chemicals

When working with chemicals in order to minimize the potential impact, observe the necessary precautions (use of gloves, masks and aprons) in accordance with the manufacturer's requirements / recommendations for handling various types of chemicals.

Proper labeling of all hazardous chemicals, for example, flammable and combustible materials, oxidants, poisonous substances, to clearly identify risks and take precautions.

A matrix of selection, use and maintenance of personal protective equipment will be prepared to prevent direct contact with corrosive, carcinogenic and irritating substances.

Appropriate leak detection procedures will be developed for different types of hazardous materials.

First aid trainings will be organized for all employees.

All safety data sheets for chemical reagents and instructions for handling hazardous substances must be prepared.

Zhappar N.,

Shaykhutdinov V.

Explanations throughout the work of the project and beyond.

Storage of chemicals

A chemical separation procedure will be developed and implemented in accordance with their classification and compatibility criteria.

Will be prepared a procedure for storing minimum stocks for each type of hazardous chemicals.

Will be provided separate storage of empty containers and containers with chemicals.

For workers will be organized a training program on the proper storage of hazardous chemicals and their health effects.

Zhappar N.,

Shaykhutdinov V.

Explanations throughout the work of the project and beyond.

The process of obtaining biopreparation

Utilization of chemicals

Hazardous chemicals / wastes will be separated at the place of their receipt, be properly processed and stored in a separate container.

An appropriate waste collection and disposal system will be identified.

Trainings for waste collection and disposal will be organized for laboratory staff.

Zhappar N.,

Shaykhutdinov V.

Explanations throughout the work of the project and beyond.

At all stages of the sub-project

Fires and explosions

New technologies will be used (smoke sensors, thermoelectric elements and fire alarm, in accordance with the requirements).

Zhappar N.,

Shaykhutdinov V.

Explanations throughout the work of the project and beyond.

Sustainable development principles application

Will be taken measures to rationally use water to reduce its consumption.

Will be taken measures to save electricity.

For the laboratory staff will be conducted classes and motivational activities on the rational use of water and electricity.

Zhappar N.,

Shaykhutdinov V.

Explanations throughout the work of the project and beyond.

Packing of finished products

Emissions to the atmosphere

For the personnel will be conducted trainings and briefings on methods to minimize emissions to the atmosphere.

Zhappar N.,

Shaykhutdinov V.

Explanations throughout the work of the project and beyond.

Biopreparation storage

A procedure will be prepared for the storage of optimal stocks in respect of the biopreparation

Appropriate storage criteria will be determined.

For employees will be organized a training program on proper methods of storing the biopreparation and their effect on health.

Explanations throughout the work of the project and beyond.

Packing of finished products

Handling of products

When packaging in order to minimize the potential impact, observe the necessary precautions (use of gloves, masks and aprons) in accordance with the requirements / recommendations of the manufacturer for handling products.

Proper labeling to clearly identify risks and take precautions.

Zhappar N.,

Shaykhutdinov V.

Explanations throughout the work of the project and beyond.

4.2. The monitoring plan for 2018-2020.

Control over the norms of emissions of harmful substances into the atmosphere is proposed to be established in accordance with the RND 211.3.01.06-87.

Responsibility for the organization of control and timely reporting is assigned to the management of the enterprise. The results of the control should be included in the reporting forms of 2TP (air) and be taken into account when assessing the activities of the enterprise.

Control sources are divided into 2 categories:

1 category. For which the condition is satisfied when Cm/MPC> 0.5 for H> 10mM / MPCmr> 0.01H or M/MPCmr> 0.1 for H <10m, as well as sources equipped with dust cleaning with an efficiency of more than 75%.

Sources of the 1st category making the greatest contribution to air pollution are controlled once a quarter.

2 category. The remaining sources are once a year.

Instrumental measurements are carried out only on the organized sources of emissions of pollutants into the atmosphere and only by an accredited laboratory.

Monitoring plan

Project work stages

What

parameter should be controlled?

Where

monitoring parameter should be monitored?

How

monitoring parameter should be monitored (what should be measured and how)?

When

the monitoring parameter should be monitored (time and frequency)?

By whom

this parameter should be tracked (responsibility)?

Instrumental control over emissions (standards according to the Permission for emissions in environment)

Boiler house, ,

SPZ

Sm / MAC not more than 0.5 for H> 10 m M / PDmm not more than 0.01N or M / MPCmr not more than 0.1 for H <10m

Observations of the level of atmospheric pollution are carried out at the posts. The observation post is a place (terrain point) where a pavilion or a car equipped with appropriate instruments is placed.

Annually, according to the schedule of the enterprise

Enterprise administration

Stages of work in the laboratory

Sharp, cutting objects

In the laboratory

(room for preparation of nutrient media)

Periodic inspection of laboratory glassware for cracks and contamination before use.

Quarterly

Zhappar N.,

Shaykhutdinov V.

Handling of chemicals

In the laboratory

Will be developed a procedure for periodic visual verification of markings, symbols and signs. Accounts will be maintained.

Authorized persons will conduct a compliance audit.

A timetable for periodic maintenance and verification of the integrity of the technical control equipment and the effectiveness of mitigation measures will be prepared.

All accidents / events will be recorded when dealing with hazardous chemicals, the records will be checked periodically by the responsible laboratory worker.

Once before starting work.

Once a year

Once before starting work.

Once before starting work.

Zhappar N.,

Shaykhutdinov V.

Chemical storage

In the laboratory

Criteria for periodic inspection and a regular visual inspection schedule will be developed.

Periodic reviews of procedures will be carried out to ensure safer working conditions with chemicals.

The authorized laboratory worker will perform periodic checks of the ventilation system.

Once before starting work.

Quarterly

Quarterly

Zhappar N.,

Shaykhutdinov V.

Disposal of chemicals and containers

In the laboratory

A proper waste collection and disposal system will be determined in accordance with the relevant regulations

In process of accumulation

Zhappar N.,

Shaykhutdinov V.

At all stages of the subproject work

Fires and explosions

At the production site and in the laboratory

Will be carried out periodic inspections of the state of fire fighting equipment.

The emergency response plan will be periodically reviewed and updated.

Quarterly

Zhappar N.,

Shaykhutdinov V.

Application of sustainable development principles

At the production site and in the laboratory

Responsible person in the field of environmental protection together with representatives of local authorities will conduct checks on the consumption of electricity and water in order to determine the current use of equipment and associated costs.

Semiannually

Zhappar N.,

Shaykhutdinov V.

Liquid waste (washing water after washing chemical dishes with synthetic detergents)

In the laboratory (washing room)

Periodic maintenance of the sewage system

Quarterly

Zhappar N.,

Shaykhutdinov V.

The strategy of reducing the risk of preparation use

On sale sites of the preparation

The responsible person for sales will consult and warn about the responsibility for using the preparation and register the examination in a special magazine

Regularly on sales

Zhappar N.,

Shaykhutdinov V.

Environmental protection activities plan

All measures for environmental protection, including monitoring, compulsory payments to the budget for the emission of carbon dioxide into the atmosphere, organization of a system for collecting, transporting and disposing of waste, excluding soil contamination with production waste, installation and maintenance of proper fire fighting equipment in the right places, safety engineering will be carried out at the expense of the basic organization of the lessor of LLP "BIOTRON GROUP"

Public hearings

The EP plan is available for public access through the following link http://www.biomedpreparat.kz/ru/ in two (Russsian and English) languages. The sub-project Commercializing bio-preparations to extend shelf-life of agricultural produce is represented at a public hearing. Date of the public hearings: September, 11, 2017 at 12.00. Venue of the public hearings: Republic of Kazakhstan, Akmolinskaya oblast, Stepnogorsk, Industrial zone 4, complex 7, telephone for inquiries 8 (71645) 3-19-85, 7-93-43. Protocol and photomaterials of the public hearings are attached.


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