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ENVIRONMENTAL MANAGEMENT PLAN

1. Mitigation measures

The following action plan for environmental mitigation contains the description of the proposed measures aimed at the reduction of a probable negative effect of the activities of the sub-project No. APP-JRG-16/0475, JRG “Implementation of the technology of cultivation and deep processing of sweet potato (Ipomoea batatas)” in the course of the following stages:

I stage – Sanitation of the initial planting material (sweet potato lines and tubers, obtained in the course of previous investigations within the framework of a signed Memorandum of Cooperation with Korea Research Institute of Bioscience and Biotechnology (KRIBB) in 2013, are available);

II stage Preparation of the field site for crop planting;

III stage Sweet potato seedling transplanting into open ground at the field site.

The plan also includes the team members responsible for accomplishment of environmental impact minimization and expenses.

Type of impact

Mitigation activities

Expenses

Responsible executives

I. Sanitation of the initial planting material under laboratory conditions

Potential for mechanical injury (cuts, bruises, etc.) as a result of misoperation with sharp and cutting objects

-Compliance with safety rules while working with a glassware in the laboratory;

- Introductory and workplace training concerning safe operation

-

Bakisheva Zh.S.

Bairon L.Zh.

Spigina T.I.

Health damages as a result of misoperation with chemical substances

- Compliance with mandatory safety measures (use of protecting gloves, safety masks, aprons) under requirements/recommendations of the manufacturer for handling of various types of chemical reagents;

- The staff will be provided with the training on the first medical aid.

-

Health damage resulting upon contact with highly flammable liquids

In case of working with highly flammable liquids, the staff should observe workplace safety rules, safety and fire safety rules

-

Noise impact

-Noise level measurement

Periodic check of the noise level which should not exceed 70 dBA (7.00-23.00)

-

Fire risk

- Compliance with the fire safety rules and regulations. Fire action rules will be developed.

- Renewal of the fire safety rules and trainings simulating emergency situations will be performed on regular basis

-

General rules

-Application of measures aimed at sustainable use and reduction of water and electric energy consumption

-

II. Preparation of the field site for crop planting

III. Sweet potato seedling transplanting into open ground at the field site

Temporary air pollution (NOx, dust, etc.) due to a high traffic of agricultural equipment

- Minimization of insignificant dust and traffic emissions by work management and adequate control at the field site;

- Application of measures aimed at minimization of a dustiness level (e.g. water spraying) during long drought periods.

-

Bakishev R.S.

Rakhimbekov A.T.

Noise impact

-Noise level measurement

Periodic check of the noise level which should not exceed 70 dBA (7.00-23.00)

-

Collection and recovery of hazardous materials (polyethylene film, chemical substance containers)

- Upon temporary storage at the field site such substances will be put into safe containers with the specification of the content, properties and handling rules of such substances.

- Containers with hazardous substances should be placed into leakproof, closable containers for the avoidance of leaks and leaching.

- Waste transport by a specialized transportation companies with the following utilization at specially allotted sites.

-

Fire risk

- Compliance with the fire safety rules and regulations. Fire action rules will be developed.

- Renewal of the fire safety rules and trainings simulating emergency situations will be performed on regular basis

-

General rules

-Application of measures aimed at sustainable use and reduction of water and electric energy consumption

-

Soil prior to planting

-Soil treatment (soil cultivation at the depth of 10-15cm, deep ploughing at the depth of a ploughed horizon of 25-30cm).

Bakishev R.S.

Rakhimbekov A.T.

Operations with soil after planting

- Soil mulching

-Mechanized protective covering of technological rows with the exact covering of the required area

Pest control measures (Annex 1 to the Environmental Management Plan)

2. Monitoring plan

With the objective of effective performance of the suggested measures aimed at environmental mitigation, inclusive of the compliance with the requirements of the environmental protection within the course of the stages of laboratory and agrotechnical activities, the following monitoring plan is developed and presented in table. This plan outlines the monitoring action plan for the sub-project Implementation of the technology of cultivation and deep processing of sweet potato (Ipomoea batatas).

Stage

Parameters

Monitoring location

Monitoring form

Monitoring Interval

Expenses

Responsible executives

I,

II, III

Risk factors while working with the chemical reagents. Safety compliance.

Laboratory

Procedure checks

On a regular basis during laboratory activities

-

Bakisheva Zh.S.

Bairon L.Zh.

Spigina T.I.

Risk factor while working with sharp, cutting objects

Compliance with mandatory safety measures

Laboratory

Procedure checks

On a regular basis during laboratory activities

-

Bakisheva Zh.S.

Bairon L.Zh.

Spigina T.I.

Liquid waste

Wash water after washing chemical ware with synthetic detergents

Laboratory

Effluent discharge into the sewerage system

Weekly

-

Bakisheva Zh.S.

Bairon L.Zh.

Spigina T.I.

Chemical waste

Collection and recovery of hazardous materials (polyethylene film, chemical substance containers)

Field site

Visual checking.

Upon temporary storage at the field site such substances will be put into safe containers with the specification of the content, properties and handling rules of such substances.

- Containers with hazardous substances should be placed into leakproof, closable containers for the avoidance of leaks and leaching.

- Waste transport by a specialized transportation companies with the following utilization at specially allotted sites.

Twice a year

-

Bakishev R.S.

Rakhimbekov A.T.

Emissions of the exhaust fumes of the machinery into atmosphere (measured in the amount of NOx content)

Monitoring points. Laboratory and field site.

Measurement of the level of exhaust fumes and checking the technical specifications of the equipment and machine.

Random measurement of the exhaust fumes

-

Spigina T.I.

Fire risk.

Compliance with the fire safety rules and regulations

Laboratory and field site

Procedure checks

Constant control

-

Operating company

Stuff health and safety

Compliance with mandatory safety measures (protecting gloves, aprons, safety masks, spectacles)

Laboratory and field site

Procedure checks

Compulsory annual medical examination of the staff members

-

Bakisheva Zh.S.

Bairon L.Zh.

Spigina T.I.

Storage conditions of chemical reagents

Fire safety compliance in accordance with the compatibility groups .Highly flammable substances should be kept in special containers.

Laboratory

Checks

Weekly

-

Bakisheva Zh.S.

Bairon L.Zh.

Spigina T.I.

Storage conditions of the final products

Temperature (15-170) and humidity (85-90%) control

Finished products warehouse

Checks

Daily

-

Bakishev R.S.

Rakhimbekov A.T.

Cultivation conditions of the sweet potato planting material

Temperature (24-250) and humidity (70%) control

Laboratory

Checks

Daily

-

Bakisheva Zh.S.

Bairon L.Zh.

Spigina T.I.

Soil requirements for planting

Gradual soil treatments (soil cultivation at the depth of 10-15cm, deep ploughing at the depth of a ploughed horizon of 25-30cm)

Field site

Control

During mulching of each row within the period of covering

-

Bakishev R.S.

Rakhimbekov A.T.

3. Development of potential and training

The local supervisory body State Institution Department of Nature Resources and environmental control of Akmoliskaya oblast. The explanatory letter concerning the absence of necessity of development of the EIA (Environment Impact Assessment) for execution of this sub-project is available.

For purposes of effective performance of environmental aspects of the sub-project and recommendations of the environmental evaluation, the training, control and execution activities will be performed by the following responsible executives of the sub-project:

Bakisheva Zh.S., Bairon L.Zh., Spigina T.I. - laboratory activities;

Rakhimbekov A.T, Bakishev R.S. agrotechnical activities.

4. Project implementation schedule and estimate of expenditure

Mitigation measures

1 year

1 quarter

2 quarter

3 quarter

4 quarter

Compliance with mandatory safety measures under requirements/recommendations of the manufacturer for handling of various types of chemical reagents

Compliance with safety rules while working with a glassware in the laboratory

Visual control of liquid wastes

First aid treatment/compulsory medical examination inspection

Mitigation measures

2 year

1 quarter

2 quarter

3 quarter

4 quarter

Compliance with mandatory safety measures under requirements/recommendations of the manufacturer for handling of various types of chemical reagents

Compliance with safety rules while working with a glassware in the laboratory

Visual control of liquid wastes

Visual control of the collection and utilization of hazardous materials

Measurement of the level of exhaust fumes and checking the technical specifications of the equipment and machine

Nose level measurement

First aid treatment/compulsory medical examination inspection

Mitigation measures

3 year

1 quarter

2 quarter

3 quarter

4 quarter

Compliance with mandatory safety measures under requirements/recommendations of the manufacturer for handling of various types of chemical reagents

Compliance with safety rules while working with a glassware in the laboratory

Visual control of liquid wastes

Visual control of the collection and utilization of hazardous materials

Measurement of the level of exhaust fumes and checking the technical specifications of the equipment and machine

Nose level measurement

First aid treatment/compulsory medical examination inspection

Capital and operational expenses, estimations and fund sources will be calculated in the course of the sup-project implementation, since this data depends on the purchased equipment and materials.

5. Integration of the Environmental Management Plan into the Project

This plan has concrete description of certain mitigation and monitoring measures, and is integrated into overall planning, conception, budget and implementation of the sub-project. Such integration will be achieved by introducing the Environmental management Plan within the framework of the project so that the plan financing and control will be executed together with other components.

6. Public hearings

Environmental management Plan is available at http://www.biomedpreparat.kz/ru/ in two (Russsian and English) languages. The sub-project Implementation of the technology of cultivation and deep processing of sweet potato (Ipomoea batatas) is represented at a public hearing. Date of the public hearings: January, 27th, 2017 at 10.00. Venue of the public hearings: Republic of Kazakhstan, Akmolinskaya oblast, Stepnogorsk, Microdistrict 9, building 3. Protocol and photomaterials of the public hearings are attached.

Environmental management plan

1.GENERAL INFORMATION ABOUT THE PROJECT AND PROJECT AREA

1.1. PROJECT DESCRIPTION

Project name: Implementation of the technology of cultivation and processing of sweet potato (Ipomoea batatas)

Project place:

Republic of Kazakhstan, Akmolinskaya oblast, Stepnogorsk

Project objective: Implementation of the technology of cultivation and processing of sweet potato (Ipomoea batatas)

Project coverage and focus area:

- Implementation of the innovation in the biotechnology field, particularly, the development and arrangement of the manufacture of the sweet potato crop.

The 1st year of the project execution will include the performance of the sanitation of the planting material of the sweet potato crop providing the optimization of the conditions of its microclonal propagation. Based on the results of these activities we will develop the methods of obtaining a sanitized planting material of the crop.

In purpose of sanitation of the initial planting material the following chemical reagents will be used: adenine, 3-indoleacetic acid (crystalline substance), kinetin, pyridoxine, B6 vitamin, thiamine, B1 vitamin, potassium phosphate, ammonium nitrate, magnesium sulphate heptahydrate, calcium chloride, potassium nitrate, sodium molibdate dehydrate, ferrous sulphate heptahydrate, sucrose, glucose, nicotinic acid, ascorbic acid, biotin, riboflavin, agar, folic acid, myo inositol, cyanocobalominun, ethylic alcohol.

Prior to the start of agrotechnical activities the soil samples will be taken at the field site for a complex microbiological and chemical examination.

Preliminary preparation of the field site in 2017 for planting the seedlings in the 2nd year of the project execution will require the performance of such agrotechnical activities with the soil as the paring, deep ploughing and harrowing.

In the beginning of 2018 we plan to start the process of the seedling propagation using the cutting method for planting into open ground.

In order to improve a previously prepared soil for planting we will perform an extra soil cultivation using a pulverizing mixer during the spring period and providing the optimal recommended air and soil temperature. After transplanting of seedlings and within the whole course of vegetation the following activities will be performed: interrow cultivation, weeding and vegetative irrigation. Prior to harvesting the vegetative part of the plants will be mechanically removed to facilitate the tuber gathering. The obtained batch of the sweet potato will be put for storage for further division into market and seed purposes. The stored batch of tubers will be checked for a preservation rate on the basis of the recommended storage conditions.

During the autumn period we will perform the agrotechnical activities for preparation of the field site for planting in 2019.

Stages of the agrotechnical activities:

1.Preparation of the field site for crop planting;

2.Soil treatment of the field site for crop planting according to the cultivation requirements of the sweet potato crop;

3. Sweet potato seedling transplanting into open ground at the field site;

4. Soil mulching.

The obtained harvest 2018 will allow us to certify the final product and participate in agricultural exhibitions for promotion and commercialization purposes.

Prior to sales the low quality tubers will be culled and standard tubers will be calibrated and packed on the automatic equipment.

In 2019 we will start the process of planting of seedling material obtained from the tubers of the previous year. Prior to the seedling transplanting into the open ground the ploughed field site will be cultivated using a pulverizing mixer to improve the structure of the soil. After transplanting of seedlings and within the whole course of vegetation the following activities will be performed: interrow cultivation, weeding and vegetative irrigation. The obtained batch of the sweet potato will be put for storage for further division into market and seed purposes. Prior to sales the low quality tubers will be culled and standard tubers will be calibrated and packed on the automatic equipment.

The activities concerning the certification of the final product will be continued and by the end of the project execution we plan to receive the Certificate of the product quality/ Also we will apply for a patent on the developed technology of cultivation.

Based on the results of the three-year tests we will publish articles in scientific journals.

Participation in agricultural exhibitions and workshops will provide a faster promotion the product for a successful commercialization of the technology.

1.2. LEGISLATION AND ADMINISTRATION

National legislation:

Code of the Republic of Kazakhstan of January, 9th, 2007 No.212-III Environmental Code of the Republic of Kazakhstan (last amended as of April, 28th, 2016) .

Code of the Republic of Kazakhstan of September, 18th,2009 on People's Health and Healthcare System (as amended up to Law of the Republic of Kazakhstan No. 159-V of January 13, 2014) about Approval of sanitary regulations Sanitary and epidemiological requirements for laboratories

1.3. PROJECT DOCUMENTATION STATUS AND LICENSE AVAILABILITY

Certificate of land or property ownership: Agreement with a household on the provision of a field site 30ha in area for crop planting between 2018-2019.

Document license type: - Environmental emission permit

Development of the Environment Impact Assessment is not required (Letter of January, the 17th, 2017 No. 01-06/179 State Institution Department of Natural Resources and Environmental Control of Akmolinskaya oblast)

Other documents and certificates: Patent of the sweet potato cultivation technology, standard and all other certificates proving the conformity and origin of the product will be developed and obtained in the course of the project execution.

2. DESCRIPTION OF THE ENVIRONMENT (BASELINE STATE)

General description of the environment at the field site of the project

Performance of the project activities in the given region is not dangerous for a human life. The project area does not belong to regions with a high seismic activity, active volcanoes, and it is not subject to floods, mudflows, hurricanes and other natural and climatic cataclysms.

Physical environment

Project site is about 180km north-east of the capital of the Republic of Kazakhstan.

The land has a flat-bottomed relief with sloping hills and rock outcrops.

Climate is extremely continental and dry. The dry, windy and disturbed weather, high daily air temperature and night frosts are typical for spring periods. Due to the influence of an intensive air warming-up in summer periods, the weather in steppe is cloudless, dry and hot. Consistent snow cover usually forms up in the middle of November to a period of 130-150 days. Frost strengthening is typical in January. The number of days with a frost weather below 25° amounts to 10-14, and sometimes to 20 days per month. The snow cover height comes to 20-22cm. Spring starts in the 2nd part of March and lasts for 1,5-2 months. Usually in the beginning of April the temperature increases up to 0. The average yearly temperature amounts to +1,3.

2 rivers Selety and Aksu- flow in the region.

The Selety river belongs to the Irtysh basin and is 407 km long. The river water is potable and is used for water supply of the nearest inhabited locality, and for inundative irrigation of bottom-land meadows, garden and farm plots. The surface of the water reservoir amounts to 36 sq. km.

The Aksu river is a small steppe river flowing towards to the Priirtyshskaya plain. At present due to artificial ponds there is no river flow. The riverbed is presented by fen, rush-grown sinkholes.

The region has a rich underground water supply resulting in formation of springs and small lakes.

Agricultural soils are liver-colored and have medium-loamy and heavy loamy mechanical composition.

The typical steppe flora is represented by herb-and-feather grass steppes with small forest outliers. The flora amounts to more than 500 plant species. Herbage plants: feather grass, wormwood, Volga fescue, salt grass and other plants. Brushwood: acacia, briar, lilac, bird cherry, currant, cherry, hawthorn. Trees: birch, linden tree, poplar, pine, aspen, willow, maple, apple-tree.

Fauna is represented by the following animals: hares, foxes, badgers, wolves, steppe polecats, corsac foxes, ground squirrels, bobac marmots, field mice, jerboas, etc. Birds: larks, swans, cranes, owls, eagles, geese, ducks, gulls, pigeons, crows, magpies, wagtails, titmice, starlings and sparrows. Insects: grasshoppers, mantids, ants, dragonflies, various butterflies. Fish: perch, bream, crucian carp, pike perch, jackfish, redeye.

Social-cultural environment

The region of the project implementation includes 11 inhabited localities: 4 settlements (Aksu, Bestobe, Zavodskoy, Shantobe) and 7 villages (Podkhoz, Karabulak, Novokronshtadka, Bogenbay, Izobilnoye, Stepnogorskoye, Kyrykkudyk).

According to the 2016 data, the region population amounts to 69093 people. More than 70 ethnic nationalities are represented as follows: Russians 32114 people (46,48%), Kazakhs 29038 people (42,03%), other nationalities 7941 people (11,49%).

The need of organization of a spare time for the stuff members is an important social-environmental problem. Thus, within the course of the project performance all the terms and conditions of the employment agreement will be observed, inclusive of the following: five-day work week, two days off, and an eight-hour work day.

Cultural and leisure environment of the project region meets the needs of all population groups. Cultural institutions of the city and settlements include 2 culture centers: CCC Gornyak and CC Miras; 2 state institutions: Local history Museum and Central Library System with 2 local and 5 settlement libraries; children’s entertainment center Play Land, central stadium, hockey courts, football grounds, play grounds, city park, mini park, central square, restaurants, cafes.

Availability of cultural and leisure services, variety of directions, forms and methods of local leisure, employment terms and working schedule meets all social and cultural requirements of the project implementation.

3. DETERMINATION OF POTENTIAL IMPACT

The process of the implementation of technology of the sweet potato cultivation and deep processing does not have significant harmful and negative impact on human life and health and environment. The following work activities will be performed in strict adherence to norms and rules of handling, storage and using of materials.

Ethyl alcohol air pollution is possible in the process of disinfection of the work zone and tools in a microbiological box during laboratory activities. Emission of chloramines fumes is possible in the process of the planting material treatment. Activities in a microbiological box involve the use of alcohol lamps which can pose danger in case of improper use. Laboratory glassware is made of thin-walled glass and may cause injures in case of breakage.

Crop planting at the field sites does not require the use of chemical protection method, since there is no pest and disease infection background.

Negative impact on the flora may arise only during execution of agrotechnical activities, particularly in the process of mixing aerobic and anaerobic microorganisms.

Polyethylene foil which is used as a mulch layer of the soil, may pollute the environment only in case of its non-removal by the end of vegetation period, but this is completely excluded owing to a compliance of the norms and rules of collection and utilization of this polyethylene foil. The remainder of the mulch foil after harvesting activities will be carefully collected from the field site and utilized at a special utilization stations.

4. MITIGATION AND MONITORING PLAN

Mitigation plan

Stages of laboratory activities

Activities

Potential environmental impact

Suggested mitigation measure

Responsible executives

Mitigation measures period

Stages of the project implementation

Sanitation of the initial planting material in laboratory conditions

Extraction of apical meristems of the sweet potato crop

- Air pollution by ethyl alcohol fumes;

- - Fire risk in case of improper handling of highly flammable liquids;

- Injuries caused by sharp objects;

- Health and safety of the staff members;

- Implementation of the air-conditioning system;

- Training on the air pollution minimization methods;

- Proper choice and installation of the fire extinguishers according to the fire safety regulations of RK of 08.02.2006;

-Periodic instructing;

- Compliance with mandatory safety measures (use of protecting gloves, safety masks, aprons, spectacles) aimed at minimization of possible contact while working with hazardous chemical substances;

- Compliance with mandatory safety measures (use of protecting rubber gloves, safety masks, aprons, spectacles);

- Compliance with safety rules while working with a glassware in the laboratory;

- Use of personal protection means for staff members;

-Records of the performed trainings concerning work place safety compliance

Bakisheva Zh.S.

Bairon L.Zh.

Spigina T.I.

I quarter 2017

IV quarter 2017

III quarter 2017

Microclonal propagation of test-tube plants of sweet potato

Testing of the planting material for presence of a viral infection (permissible parameters of the initial planting material of agricultural crop according to GOST/SAUS: severe virus is not allowed; mild virus 0,5%)

Stages of agrotechnical activities

Activities

Potential environmental impact

Suggested mitigation measure

Responsible executives

Mitigation measures period

Stages of the project implementation

Preparation of the field site for crop planting

Treatment of the field soil for crop planting in accordance with the cultivation requirements of sweet potato crop (soil treatment depth should be set to a full depth of a ploughing layer of 25-30cm; soil structure should naturally break into structural separates and aggregates)

Displacement of a oil horizon, mixing of aerobic and anaerobic microorganisms

Gradual preparation of the soil (soil cultivation to the depth of 10-15cm, deep ploughing to the depth of a ploughing horizon of 25-30cm)

Rakhimbekov A.T.

Bakishev R.S.

III quarter 2017

II quarter 2018

II quarter 2019

Sweet potato seedling transplanting into open ground at the field site

Soil mulching

Use of undecomposable polyethylene foil

Mechanized protective covering of technological rows with the exact covering of the required area

Rakhimbekov A.T.

Bakishev R.S.

II quarter 2018

II quarter 2019

Annex G: Mitigation measures during laboratory operations and international best practices for laboratory operations

Potential environmental problems

Mitigation measures

Monitoring strategies and emergency response measures

1. Atmospheric emissions

· The laboratory personnel will be provided with trainings and instructions on the methods aimed at reducing atmospheric emissions as much as possible.

Purchase of equipment, free of ozone-depleting substances (refrigerators, air conditioners, fire extinguishers, etc.) and proper maintenance of the equipment containing ozone-depleting substances.

List of sources, polluting the atmosphere and specifying a corresponding category, will be forwarded to the laboratory.

List of actual and potential waste sources in the laboratory (chimney and vent stacks, etc.)

Assessment of impact of the atmosphere polluting substances will be executed twice a year

Control system inspection will be performed on a periodic basis.

Emission registration with periodic inspection of the records by the supervisory group of the Bank and any authorized governmental body. Annual certification comes within duties of the responsible executive (Waste Management System).

Regular inspection and maintenance of the air ventilation system.

2. Disposal of sewage

A specified list of waste water sources generation and its disposal will be prepared.

Proper procedure of minimization of waste water will be developed (e.g. pre-treatment using neutralizing materials, etc.)

Use of septic tank systems or proper waste water treatment systems taking into account waste water characteristics would be an asset. After proper treatment the waste water will be disposed to municipal sewage system.

The laboratory personnel will be trained to minimize and manage the waste water disposal process.

Periodic maintenance of the sewerage system.

Periodic inspection of the laboratory procedures for compliance with the regulatory requirements.

Regular trainings on minimization of waste water.

3. Hazardous and radioactive wastes (not provided by the project)

Such various waste types as unused chemical substances, waste solvents, etc. Will be identified for special system of collection, utilization and transportation.

Special method of division and utilization of waste lead and alkaline batteries will be developed.

Training programs on safe handling of hazardous wastes will be prepared for the laboratory personnel.

Waste minimization procedure will be developed integrated.

Assessment of impact of hazardous and radioactive wastes will be executed twice a year.

Medical examination for all laboratory workers will be performed 4 times a year.

Formation and utilization of wastes will be recorded, the laboratory personnel will perform regular inspections of the records.

4. Handling of the hazardous chemical substances

Compliance with mandatory safety measures (use of protecting gloves, safety masks, aprons) under requirements/recommendations of the manufacturer for handling of various types of chemical substances.

Proper marking of all hazardous substances, for example, flammable materials, oxidants, poisonous substances for exact risk identification and safeguarding.

Matrix of choice, use and maintenance of the personal protection means for prevention of a direct contact with corrosive, cancerogenic and irritating substances.

Proper exhaust ventilation system for prevention of the impact of fumes and vapours of hazardous chemical substances will be designed during the reconstruction operations.

Proper radioactive protection means will be purchased and used during operations with radioactive substances.

Proper procedures on leak localization will be developed for various types of hazardous materials.

First aid measures training will be arranged for the personnel.

Training on the handling of hazardous chemical substances will be performed among the personnel. Instructor training program will be developed.

Periodic assessment of the impact of chemical substances. All staff members will have periodic medical examination.

Procedure on the periodic visual inspection of marking, symbols and signs will be developed. All the corresponding records will be kept.

Authorized executives will execute the inspection non the compliance with the regulatory requirements.

Diagram of periodic maintenance and check-out of the equipment, technical control and efficiency of the mitigation measures will be prepared.

Registration of all incidents during working with hazardous chemical substances will be performed. The records will be periodically checked by a responsible executive of the laboratory.

5. Storage of hazardous chemical substances

Development and implementation of the procedure of the chemical substance division in accordance with the classification and compatibility criteria.

Preparation of the storage procedure of minimum reserve concerning each type of hazardous chemical substances.

Determination of proper storage criteria for flammable and volatile chemical substances. Separate storage of empty containers and containers with chemical substances will be provided.

Proper exhaust ventilation system for prevention of the impact of fumes and vapours of hazardous chemical substances will be designed during the reconstruction operations.

Training on proper storage of hazardous chemical substances and their health effect will be arranged for the personnel.

Criteria for periodic inspection and regular visual control diagram will be developed.

Periodic re-examination of the procedures will be performed for provision of safe working conditions with high toxic, cancerogenic, chemically active and mutagenic materials, if applicable.

Authorized laboratory member will perform periodic inspections of the air ventilation system.

6. Utilization of hazardous chemical substances

Hazardous chemical substances/wastes will be identified at the place of receipt, properly used and stored in a separate container.

Proper waste collection and utilization system will be determined.

Training on waste collection and utilization procedures will be arranged for the laboratory personnel.

Local environmental bodies will perform periodic monitoring of the waste collection and utilization procedures (to be agreed)

7. Fires and explosions

Proper fire protection equipment should be purchased and installed in the appropriate places. New technologies will be used (smoke sensors, thermoelectric elements and fire alarm system in accordance with the requirements).

Periodic inspections of the fire-fighting equipment status.

Emergency response plan will be periodically reviewed and renewed.

8. Implementation of the sustainable development principles

Development of measures aimed at water management.

Development of measures aimed at electric energy management.

Activities aimed at water and electric energy management will be arranged for the laboratory personnel.

Inspection of the water and electric energy consumption and corresponding expenses by the authorized laboratory member with representatives of local authorities.

Monitoring plan

Monitoring stages

1. Sanitation of the initial planting material in laboratory conditions

Monitoring parameter

Place of the monitoring parameter

Method of parameter monitoring

Period of monitoring

Responsible executives

Stages of the project implementation

1.1Extraction of apical meristems of the sweet potato crop

1.2.Microclonal propagation of test-tube sweet potato plants

1.3 Testing of the planting material on presence of viral infection

Air

(forced removal of hazardous substances from the work zone via HEPA filters and emission into external exhaust system)

Laboratory

Periodic check and maintenance of the air ventilation system

Assessment of the air polluting agents occurrence will be performed twice a year

Spigina T.I.

Sharp, cutting objects

Laboratory

(room for preparation of the nutrient media)

Periodic inspection of the laboratory glassware for presence of cracks and impurities prior to use

Quarterly

Bakisheva Zh.S.

Hazardous chemicals ethyl alcohol

Laboratory (storage warehouse)

- Compliance with the minimum procedures on inventory and storage of each hazardous substance

-Proper marking of all hazardous chemical substances, for example, flammable substances, oxidants, toxic substances, for exact risk identification and safeguarding.

- Preparation of the matrix for choice, use and maintenance of the personal protection means for prevention of a direct contact with caustic , cancerogenic, and irritating substances.

Quarterly

Bairon L.Zh.

Spigina T.I.

Storage of minimum reserve concerning each type of hazardous chemical substances

Storage of ethyl alcohol in metal tanks of different capacity and forms with the measurement capability of the volume of ethyl alcohol

Bairon L.Zh.

Spigina T.I.

Liquid waste

(wash water after washing chemical ware with synthetic detergents)

Laboratory

(washing room)

- Periodic maintenance of the sewage collecting system.

Quarterly

Bairon L.Zh.

Spigina T.I.

Fires and explosions

Laboratory

Periodic checking of the fire-extinguishing and fire safety equipment

Periodic improvement of the emergency response

Bairon L.Zh.

Spigina T.I.

Utilization of hazardous chemical substances/ wastes

- Hazardous chemical substances/wastes will be identified at the place of receipt, properly used and stored in a separate container.

-Proper waste collection and utilization system.

Bairon L.Zh.

Spigina T.I.

Stages of agrotechnical activities

Monitoring parameter

Place of the monitoring parameter

Method of parameter monitoring

Period of monitoring

Responsible executives

Preparation of the field site for crop planting

Soil ( treatment depth should be set to a full depth of a ploughing layer of 25-30cm; soil structure should naturally break into structural separates and aggregates)

Field site

Treatment depth using a scale

Minimum once a shift of an operator during the soil treatment period

Rakhimbekov A.T.

Bakishev R.S.

Sweet potato seedling transplanting into open ground at the field site

Soil ( treatment depth should be set to a full depth of a ploughing layer of 25-30cm; soil structure should naturally break into structural separates and aggregates)

Field site

Inspection of the exact covering of the required area with a polyethylene foil

During the row mulching within a period of covering

Rakhimbekov A.T.

Bakishev R.S.

Storage conditions of materials and chemical substances

Laboratory

- Periodic check

-Visual inspection on a regular basis

Weekly

Bairon L.Zh.

Spigina T.I.

Storage conditions of final products

Final products storage

- Temperature and humidity conditions

Weekly

Rakhimbekov A.T.

Bakishev R.S.

Sanitation conditions of the storage

Storage and working room

-Storage sanitation conditions

Weekly

Rakhimbekov A.T.

Bakishev R.S.

ENVIRONMENTAL MANAGEMENT PLAN IN RESPECT OF MATERIALS

Sub-project beneficiary

OrganicFood LLP

Project name

Implementation of the technology of cultivation and deep processing of sweet potato (Ipomoea batatas)

Project work scope project description

The objective of the sub-project is the implementation of the technology of cultivation and deep processing of sweet potato (Ipomoea batatas) in RK.

Main sub-project tasks:

- Propagation of the sweet potato planting material;

- Purchase of the technological equipment;

- Installation of irrigation system for the sweet potato seedling in field conditions;

- Cultivation of planting material for sales within RK;

- Product promotion within domestic and foreign markets and import substitution.

Implementation of the technology of cultivation of various sweet potato cultivars for RK regions and obtaining a pilot batch of sweet potato will be performed as a result of the sub-project.

Fields of application:

- In agricultural sector the tubers and tops of sweet potato are used as fodder for agricultural animals (cattle, pigs, and poultry). Also a green mass of sweet potato is used for silage and hay production.

- Sweet potato is used as a food crop both raw and cooked, and green leaves are used in salads.

- Sweet potato can be processed into flour, molasses, starch, glucose, etc.

Organization support of the project/ supervision of the project implementation

State Institution Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan

Potential environmental impact of the project

The process of the implementation of technology of the sweet potato cultivation and deep processing does not have significant harmful and negative impact on human life and health and environment. The following work activities will be performed in strict adherence to norms and rules of handling, storage and using of materials.

Ethyl alcohol air pollution is possible in the process of disinfection of the work zone and tools in a microbiological box during laboratory activities. Emission of chloramines fumes is possible in the process of the planting material treatment. Activities in a microbiological box involve the use of alcohol lamps which can pose danger in case of improper use. Laboratory glassware is made of thin-walled glass and may cause injures in case of breakage.

Negative impact on the flora may arise only during execution of agrotechnical activities with the use of agricultural machinery.

Crop planting at the field sites does not require the use of chemical protection method, since there is no pest and disease infection background. Thus, there will be no negative chemical effect on the soil.

The harvesting process has a negative effect in the form of pressure of agricultural machinery on the soil.

In the process of culling, calibration and packing of the final product the mechanical damages of the product will be caused by operating mechanisms of automated equipment.

TRIALS/INVESTIGATIONS/INSTALLATION

INVESTIGATIONS

Describe the investigation stages

1.Sanitation of the initial planting material:

1.1 Working out the technology of obtaining a sanitized sweet potato material. Development of the methods of obtaining a sanitized material.

1.2 Optimization of microclonal propagation conditions

1.3 Testing of the planting material for presence of viral infection. Determination of optimal methods for viral load testing

1.4 Preparation of the experimental site for crop planting

1.5 Increase of the amount of test-tube plants by microclonal propagation method. (5000 cuttings)

2. Agrotechnical operations for preparation of the field site for planting of the next year crop

2.2 Execution of a complex microbiological and chemical analysis of the soil samples from the field site.

3. Investigation of the influence of abiotic factors of natural and climatic conditions for determination of a potential crop yield of sweet potato under microplot tests:

3.1 Propagation of seedlings by cutting method (20000 cuttings)

3.2 Preparation of the experimental site for planting according to the requirements of the sweet potato crop cultivation.

3.3 Seedling transplanting into open ground. Estimation of the survival rate of the planting material. Working out the agrotechnical activities for optimization of the cultivation conditions of sweet potato in open ground.

3.4 Harvesting.

3.5 Working out the storage technology of sweet potato seed material in accordance with the requirements. Estimation of the product preservation.

4. Activities on the product certification.

5. Participation in agricultural exhibitions, arrangement of commercial sales.

6. Investigation of the growth and development characteristics of the sweet potato crop using the culticvation technology on a scale:

6.1 Seed propagation by the cutting method (40000 cuttings).

6.2 Complex microbiological and chemical analysis of the soil samples from the pilot field site. Preparation of the pilot field site for planting according to the requirements of the sweet potato crop cultivation. Seed transplanting into propagation nursery garden. Assessment of the seed material survival rate.

6.3 Compliance with the technological parameters of the sweet potato crop cultivation.

6.4 Collection and estimation of the obtained yield and analysis of the yield structure. Agrotechnical activities on the pilot field site during post-harvesting period. Filing for a patent on the developed technology. Publication of the article based on the results of the performed investigations.

7. Product certification activities.

8. Participation in agricultural exhibitions. Arrangement of commercial sales.

Special wastes in the course of the investigation project

None

Sharp, cutting objects [all sharp objects which may cause a sliver (followed by a contagious infection or not) inclusive of hypodermic needles, surgical needles, luer connectors, scalpels, knives, razor blades, glass droppers, broken glass, etc.]

Glass droppers and broken glass

Hazardous biological wastes [organism liquids, blood, organs, tissues, culture dishes, glass slides for microbiological investigations, cover slides, etc. ]

None

Radioactive wastes [solid, liquid and gaseous wastes, contaminated with radionuclides and radioactive isotopes]

None

Hazardous chemical wastes [any substance, liquid or solid, characterized by at least one of the following properties: explosiveness, flammability, toxicity, corrosive effect, local abrasion, chemical activity, genotoxicity (cancerogenic, mutagenic, teratogenic properties) inclusive of cytotoxic agents. Also all the containers used for storage of such substances]

Polyethylene foil; Containers of the chemical substances with the specification of the content and properties, and the information on handling of such substances.

Animal test commission

None

Used water

Washing of the laboratory glassware and vegetative irrigation

Toxic substances

None

Atmospheric emissions

None

Other information

None

APPROVAL DOCUMENTS

Required permits for project preparation/investigation performance[1]

Available:

- The local supervisory body State Institution Department of Nature Resources and environmental control of Akmoliskaya oblast. The explanatory letter concerning the absence of necessity of development of the EIA (Environment Impact Assessment) for execution of this sub-project is available.

The following document will be obtained in the course of the project :

- Certificate of accreditation as a n entity of scientific and (or) scientific-and-technical activities

- Patent on the technology of cultivation of the sweet potato crop, Standard and all Certificates proving conformance and origin of the product.

LIST OF MATERIALS

List of materials/ chemical substances which will be used during the project

If available, put CAS[2] No. Corresponding to a material/ chemical substance ,[3]

Does it belong to hazardous materials according to the Chemical Substance Law?

Specify the material category according to the Chemical Substance Law (flammable, toxic, etc.)

Adenine hydrochloride hydrate

2922-28-3

No

Does not belong to any category

3-Indoleacetic acid, crystalline substance

87-51-4

No

Does not belong to any category

Kinetin

525-79-1

No

Does not belong to any category

Pyridoxine

58-56-0

No

Does not belong to any category

B1 iamine

67-03-8

No

Does not belong to any category

KH2PO4

Potassium phosphate

7778-77-0

No

Does not belong to any category

NH4NO3

Ammonium nitrate

64-84-52-2

No

Does not belong to any category

MgSO4*7H2O

Magnesium sulphate heptahydrate

10034-99-8

No

Does not belong to any category

CaCl2

Calcium chloride

10043-52-4

No

Does not belong to any category

KNO3

Potassium nitrate

7757-79-1

No

Does not belong to any category

Na2MoO4*2H2O

Sodium molibdate dehydrate

10102-40-6

No

Does not belong to any category

FeSO4*7H2O

Ferrous sulphate heptahydrate

7782-63-0

No

Does not belong to any category

Sucrose

57-50-1

No

Does not belong to any category

Glucose

50-99-7

No

Does not belong to any category

3

Nicotinic acid

59-67-6

No

Does not belong to any category

Ascorbic acid

50-84-7

No

Does not belong to any category

Biotin

58-85-5

No

Does not belong to any category

Riboflavin

83-88-5

No

Does not belong to any category

Agar

9002-18-0

No

Does not belong to any category

Folic acid

59-30-3

No

Does not belong to any category

My inositol

87-89-8

No

Does not belong to any category

12

Cyanocobalominum

68-19-9

No

Does not belong to any category

Ethyl alcohol

64-17-5

No

Highly flammable liquid

Safety passports of the chemical reagents are attached.

Annex 1 to the Environmental Management Plan

Of the sub-project No. APP-JRG-16/0475, JRG

Implementation of the technology of cultivation and

deep processing of sweet potato (Ipomoea batatas)

Pest Control Plan

1. Initial data

i) Plan objective

Development of effective protection activities against pests, diseases and weeds in compliance of the environmental balance and application of agrotechnical, mechanical and cultural methods of integrated protection.

ii) Pest Control Program target

Use of alternative measures of the implementation of chemical pesticides and minimization of the environmental negative impact.

2. Integrated Pest Control

2.1 Integrated pest control principles:

Management of the phytosanitary state of ecosystems using various methods of plant protection aimed at a phytosanitary well-being of the territory.

a) Agrotechnical control.

This method demonstrates the protection against weeds. Weed control activities at the field site start a year ahead of the seedling transplanting into open ground. The soil will be pre-cultivated to the depth of 10-12cm for destruction of the weed root layer. This will induce the germination of the seeds on the soil surface. Following the renewal of the weed growth on the cultivated field, the treatment depth will be increased to the depth of a ploughing horizon (25-27cm). The final pre-planting treatment will include rotary cultivation of the soil to the depth of 15-18cm with resulting aggregated soil structure.

The following weed control will include mechanical weeding of interrow spaces.

b) Mechanical control

Plant protection practice has a limited application of this method owing to its labor inputs, but in this case, due to small cultivation areas and slight potential pest contagion centers, this method will be effective.

This method will be used in direct physical pest extermination by collecting pests. These activities will be performed during morning and evening hours due to their slow-moving state within these periods.

c) Cultural control

Phytoweeding activities will be performed within the whole vegetation course and growth and development course for purposes of determination and culling of non-conforming plants. 3 and more phytoweeding activities will be executed per season so that to exclude the non-conforming products by the start of the harvesting operations. The main target of this work is to obtain a healthy yield without visible and latent diseases and damages.

Crop rotation and introduction of other crops will allow to control the accumulation of the soil infection in the form of the disease spores and hibernating stages of the major crop insects. Rotating crops at the field site may include: spring species of grain crops, annual grasses, bean cultures and clean fallow. Crop choice will depend on many factors, but the main points will be the economic attractiveness and specialized agricultural equipment status.



[1] All permits should be attached to the final documentation

[2] Chemical Abstract Service No.

[3] Material safety data sheets should be attached to the final documentation

.